Freeform Bioprinting of Liver Encapsulated in Alginate Hydrogels Tissue Constructs for Pharmacokinetic Study

An in vitro model that can be realistically and inexpensively used to predict human response to various drug administration and toxic chemical exposure is needed. By fabricating a microscale 3D physiological tissue construct consisting of an array of channels and tissue-embedded chambers, one can selectively develop various biomimicking mammalian tissues for a number of pharmaceutical applications, for example, experimental pharmaceutical screening for drug efficacy and toxicity along with apprehending the disposition and metabolic profile of a candidate drug. This paper addresses issues relating to the development and implementation of a bioprinting process for freeform fabrication of a 3D cell-encapsulated hydrogel-based tissue construct, the direct integration onto a microfluidic device for pharmacokinetic study, and the underlying engineering science for the fabrication of a 3D microscale tissue chamber as well as its application in pharmacokinetic study. To this end, a prototype 3D microfluidic tissue chamber embedded with liver cells encapsulated within a hydrogel matrix construct is bioprinted as a physiological in vitro model for pharmacokinetic study. The developed fabrication processes are further validated and parameters optimized by assessing cell viability and liver cell phenotype, in which metabolic and synthetic liver functions are quantitated.Mechanical Engineerin

Making Scholarly Activity Available to the Masses: The Scaffolding of Scholarship Throughout the Undergraduate Curriculum

Florida Gulf Coast University’s Quality Enhancement Plan (QEP) focuses on improving student critical thinking, information literacy, and written communication. Rather than developing these skills through traditional methods (e.g., through senior-level, independent research), these learning outcomes are practiced through scholarly experiences. Traditional undergraduate scholarship manifests itself through terminal, senior capstone or research experiences. These, because of the economy of scale, typically reach a minority of students, often just honors students or those approached by faculty mentors. At FGCU, however, scholarly experiences are a part of the curriculum throughout the program of study, and scaffolded to build greater depth and sophistication. Presented here are examples from both a program in STEM (Marine Science) and the humanities (Music Performance). Students in Marine Science receive their first exposure to the vetting of literature and expository scientific writing within their general education science courses. Students are presented with an exercise to evaluate the credibility of web-based literature using the CRAAP test. A semester-long writing assignment has them investigate an earth-process-related problem that has societal consequences. They review and evaluate the secondary literature, prepare a first draft that is critiqued, and then submit a final version while meeting a number of milestones along the way. Students enter the major’s curriculum through a course entitled “Scientific Process”, which introduces them to all aspects of scientific research and culminates with them writing and defending a research proposal they may eventually work to completion. Numerous courses at the upper-class level are designed as scholarly focused or enriched, a branding requiring that certain criteria are met. In these courses, students often participate in genuine collaborative research projects that can lead to student publication and enhance faculty productivity. Finally, as a senior, the capstone course requires that they produce a scholarly poster or oral presentation that is either given in the class or within a university forum. Music Performance students’ experiences track towards demonstration of content mastery in the artifact of a senior recital. In this public display of scholarly achievement a student presents repertoire from major historical eras on his or her instrument or voice for an hour or more. Additionally the students complete a comprehensive document analyzing music in terms of performance practice (how and why certain music should be performed to meet historically appropriate creations and recreations). Students enter this major their freshman year after an audition process and immediately begin developing the skills required to demonstrate proficiency as professional musicians. Experiences performing in ensembles and in private lessons cultivate listening skills to make informed musical judgments. Theory courses develop students’ abilities to hear music with their eyes. Upper level courses require students to clearly articulate in writing their thoughts about music’s formal properties, why certain music requires particular performance considerations, and how to execute those performance requirements in their technique. The conundrum for collection of data is how to assess university-wide learning outcomes in the context of a performance. Without a tangible artifact, FGCU relies on artist teams to develop assessment procedures that accurately capture if students meet targets as demonstrated in performance. Though too early for us to have extensive assessment data, anecdotal evidence suggests students enjoy this approach and are honing their skills within these learning outcomes. We anticipate these improvements will increase graduates’ life-long learning potential, as well as their competitiveness for employment and further education

Cyclin-Dependent Kinase-Like Function Is Shared by the Beta- and Gamma- Subset of the Conserved Herpesvirus Protein Kinases

The UL97 protein of human cytomegalovirus (HCMV, or HHV-5 (human herpesvirus 5)), is a kinase that phosphorylates the cellular retinoblastoma (Rb) tumor suppressor and lamin A/C proteins that are also substrates of cellular cyclin-dependent kinases (Cdks). A functional complementation assay has further shown that UL97 has authentic Cdk-like activity. The other seven human herpesviruses each encode a kinase with sequence and positional homology to UL97. These UL97-homologous proteins have been termed the conserved herpesvirus protein kinases (CHPKs) to distinguish them from other human herpesvirus-encoded kinases. To determine if the Cdk-like activities of UL97 were shared by all of the CHPKs, we individually expressed epitope-tagged alleles of each protein in human Saos-2 cells to test for Rb phosphorylation, human U-2 OS cells to monitor nuclear lamina disruption and lamin A phosphorylation, or S. cerevisiae cdc28-13 mutant cells to directly assay for Cdk function. We found that the ability to phosphorylate Rb and lamin A, and to disrupt the nuclear lamina, was shared by all CHPKs from the beta- and gamma-herpesvirus families, but not by their alpha-herpesvirus homologs. Similarly, all but one of the beta and gamma CHPKs displayed bona fide Cdk activity in S. cerevisiae, while the alpha proteins did not. Thus, we have identified novel virally-encoded Cdk-like kinases, a nomenclature we abbreviate as v-Cdks. Interestingly, we found that other, non-Cdk-related activities reported for UL97 (dispersion of promyelocytic leukemia protein nuclear bodies (PML-NBs) and disruption of cytoplasmic or nuclear aggresomes) showed weak conservation among the CHPKs that, in general, did not segregate to specific viral families. Therefore, the genomic and evolutionary conservation of these kinases has not been fully maintained at the functional level. Our data indicate that these related kinases, some of which are targets of approved or developmental antiviral drugs, are likely to serve both overlapping and non-overlapping functions during viral infections

Methods for Volumetric Reconstruction of Visual Scenes

In this paper, we present methods for 3D volumetric reconstruction of visual scenes photographed by multiple calibrated cameras placed at arbitrary viewpoints. Our goal is to generate a 3D model that can be rendered to synthesize new photo-realistic views of the scene. We improve upon existing voxel coloring/space carving approaches by introducing new ways to compute visibility and photo-consistency, as well as model infinitely large scenes. In particular, we describe a visibility approach that uses all possible color information from the photographs during reconstruction, photo-consistency measures that are more robust and/or require less manual intervention, and a volumetric warping method for application of these reconstruction methods to large-scale scenes

A categorical foundation for Bayesian probability

Given two measurable spaces $H$ and $D$ with countably generated $\sigma$-algebras, a perfect prior probability measure $P_H$ on $H$ and a sampling distribution $S: H \rightarrow D$, there is a corresponding inference map $I: D \rightarrow H$ which is unique up to a set of measure zero. Thus, given a data measurement $\mu: 1 \rightarrow D$, a posterior probability $\widehat{P_H}= I \circ \mu$ can be computed. This procedure is iterative: with each updated probability $P_H$, we obtain a new joint distribution which in turn yields a new inference map $I$ and the process repeats with each additional measurement. The main result uses an existence theorem for regular conditional probabilities by Faden, which holds in more generality than the setting of Polish spaces. This less stringent setting then allows for non-trivial decision rules (Eilenberg--Moore algebras) on finite (as well as non finite) spaces, and also provides for a common framework for decision theory and Bayesian probability.Comment: 15 pages; revised setting to more clearly explain how to incorporate perfect measures and the Giry monad; to appear in Applied Categorical Structure